In enzymology, a carboxy-cis,cis-muconate cyclase (EC 5.5.1.5) is an enzyme that catalyzes the chemical reaction

3-carboxy-2,5-dihydro-5-oxofuran-2-acetate ⇌ {\displaystyle \rightleftharpoons } 3-carboxy-cis,cis-muconate

Hence, this enzyme has one substrate, 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate, and one product, 3-carboxy-cis,cis-muconate.

This enzyme belongs to the family of isomerases, specifically the class of intramolecular lyases. The systematic name of this enzyme class is 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate lyase (decyclizing). This enzyme is also called 3-carboxymuconate cyclase. This enzyme participates in benzoate degradation via hydroxylation.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1JOF.

References

  • GROSS SR, GAFFORD RD, TATUM EL (1956). "The metabolism of protocatechuic acid by Neurospora". J. Biol. Chem. 219 (2): 781–96. doi:10.1016/S0021-9258(18)65737-0. PMID 13319299.



Muconate cycloisomerase Proteopedia, life in 3D

Carbocisteine Molecular Structure, Mucolytic, Ball and Stick 3d Model

The Structure of a New Crystal Form of Muconate Lactonizing Enzyme

Figure 4 from An expanded enzyme toolbox for production of cis, cis

Figure 1 from cis,cisMuconic acid isomerization and catalytic